We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Screening Program Prevents Transfusion-Transmitted Babesiosis

By LabMedica International staff writers
Posted on 20 Aug 2012
Print article
A laboratory-based blood donor-screening program for Babesia microti has been developed and implemented to reduce transfusion-transmitted babesiosis (TTB).

Selective B. microti donor screening was performed using real-time polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA) to reduce the incidence of TTB in neonates and pediatric sickle cell and thalassemia patients.

A team of scientists collaborating with the Rhode Island Blood Center (Providence, RI, USA) screened 2,113 units of blood from 1,783 eligible donors for the presence of B. microti between July 8, 2010, and June 30, 2011. B. microti antibody testing was performed at Imugen, Inc. (Norwood, MA, USA) using an IFA method with additional modifications to improve sensitivity and specificity and to facilitate future high-throughput screening. PCR was carried out employing primers and probes targeting the B. microti 18S ribosomal ribonucleic acid (RNA) gene and was also performed at Imugen, Inc., using a highly sensitive and specific real-time fast PCR method approved by the US Food and Drug Administration (FDA; Silver Springs, MD, USA).

There were 2,113 units tested with 2,086 negative results, 26 positive IFA results (1.23%), and one indeterminate PCR result (0.05%). No reported case of TTB occurred with any B. microti–screened unit transfused to the targeted patients (0/787 units) or to any patient who received the screened units (0/2,086 units). Before screening, there were seven cases of TTB in neonates, sickle cell, and thalassemia patients from 6,500 unscreened units (one case/929 units) and 24 cases in the total transfused population from 496,545 units distributed (one case/20,686 units).

The authors concluded that the problem of donor loss due to laboratory screening would be outweighed by the benefit of increased protection such screening provides. After three neonatal TTB cases occurred from one infected donor in 2006, the hospital began transfusing one red blood cell unit per patient with up to 10 aliquots per unit. The hospital with screening could safely use one unit for many neonates. The prescreening TTB incidence data of 1 in 995 aliquots compared to the postscreening TTB incidence of 0 in 545 aliquots are promising. Therefore, donor testing mitigated TTB in a highly vulnerable recipient population.

Babesia microti, the most common cause of human babesiosis, is also the most frequently reported transfusion-transmitted microbial pathogen in the United States with 159 cases of transfusion-associated infection reported during 1979 to 2009, involving 19 states. Rhode Island is highly endemic for babesiosis with rates of reported babesiosis exceeding 90 cases per 100,000 in 2008. The study was published in the July 2012 edition of the journal Transfusion.

Related Links:
Rhode Island Blood Center
Imugen, Inc.
US Food and Drug Administration


Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
Specimen Collection & Transport
POCT Fluorescent Immunoassay Analyzer
FIA Go
New
Gold Member
TORCH Panel Rapid Test
Rapid TORCH Panel Test

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Molecular Diagnostics

view channel
Image: MOF materials efficiently enrich cfDNA and cfRNA in blood through simple operational process (Photo courtesy of Science China Press)

Blood Circulating Nucleic Acid Enrichment Technique Enables Non-Invasive Liver Cancer Diagnosis

The ability to diagnose diseases early can significantly enhance the effectiveness of clinical treatments and improve survival rates. One promising approach for non-invasive early diagnosis is the use... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.