We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
INTEGRA BIOSCIENCES AG

Download Mobile App




Events

09 Apr 2024 - 12 Apr 2024
15 Apr 2024 - 17 Apr 2024
23 Apr 2024 - 26 Apr 2024

Immunodiagnostic Test Predicts Progression of Pancreatic Cyst to Cancer

By LabMedica International staff writers
Posted on 17 Jun 2019
Print article
In this photomicrograph, the brown areas were stained for a biomarker in tissue from a patient who developed pancreatic cancer from a cyst (Photo courtesy of Dr. Koushik Das, Washington University).
In this photomicrograph, the brown areas were stained for a biomarker in tissue from a patient who developed pancreatic cancer from a cyst (Photo courtesy of Dr. Koushik Das, Washington University).
By using an immunodiagnostic test to detect the Das-1 antibody biomarker in fluid from pancreatic cysts, researchers were able to identify those cysts that were likely to become cancerous with 95% accuracy.

Although pancreatic cystic lesions (PCLs) are frequently and incidentally detected, it has been difficult to determine their risk of malignancy. Previous studies had indicated that in immunohistochemical and ELISA analyses of tissue and cyst fluid from pancreatic intraductal papillary mucinous neoplasms (IPMNs), the monoclonal antibody Das-1 identified those at risk for malignancy with high levels of specificity and sensitivity. Therefore, investigators at Washington University (St. Louis, MO, USA) used pancreatic cyst samples from a multicenter cohort to validate the ability of Das-1 to identify high-risk PCLs, in comparison to clinical guidelines and clinical features.

For this study, the investigators obtained cyst fluid samples of 169 PCLs (90 IPMNs, 43 mucinous cystic neoplasms, and 36 non-mucinous cysts) from patients undergoing surgery at four tertiary referral centers. Histology findings from surgical samples, analyzed independently and centrally re-reviewed in a blinded manner, were used as the reference standard. An ELISA with Das-1 was performed in parallel using banked cyst fluid samples.

The results of the analyses revealed that the ELISA for Das-1 identified high-risk PCLs with 88% sensitivity and 99% specificity, and 95% accuracy, at a cut-off optical density value of 0.104. In 10-fold cross validation analysis with 100 replications, Das-1 identified high-risk PCLs with 88% sensitivity and 98% specificity.

“Some cysts have the potential to become pancreatic cancer, so there is the thought that we should err on the side of caution and remove the cysts,” said first author Dr. Koushik K. Das, assistant professor of medicine at Washington University. “But pancreas surgery is complicated. It often requires removal of the spleen, portions of the stomach, small intestine, and bile duct. In an ideal world, we only would do surgery on people whose pancreatic cysts are likely to develop into cancer. As it is, we probably do not operate on some people who need surgery and sometimes do operate when cancer is not present because we are working with imprecise information. Many cysts, if not most, probably should be left alone. But we do that at our peril because we may miss individuals harboring cancer. If we had a better biomarker, we would not have to rely on imperfect clinical and radiographic information.”

The Das-1 ELISA study was published in the June 5, 2019, online edition of the journal Gastroenterology.

Related Links:
Washington University

Platinum Member
COVID-19 Rapid Test
OSOM COVID-19 Antigen Rapid Test
One Step HbA1c Measuring System
GREENCARE A1c
Complement 3 (C3) Test
GPP-100 C3 Kit
Gold Member
ADAMTS-13 Protease Activity Test
ATS-13 Activity Assay

Print article

Channels

Clinical Chemistry

view channel
Image: Reaching speeds up to 6,000 RPM, this centrifuge forms the basis for a new type of inexpensive, POC biomedical test (Photo courtesy of Duke University)

POC Biomedical Test Spins Water Droplet Using Sound Waves for Cancer Detection

Exosomes, tiny cellular bioparticles carrying a specific set of proteins, lipids, and genetic materials, play a crucial role in cell communication and hold promise for non-invasive diagnostics.... Read more

Hematology

view channel
Image: The low-cost portable device rapidly identifies chemotherapy patients at risk of sepsis (Photo courtesy of 52North Health)

POC Finger-Prick Blood Test Determines Risk of Neutropenic Sepsis in Patients Undergoing Chemotherapy

Neutropenia, a decrease in neutrophils (a type of white blood cell crucial for fighting infections), is a frequent side effect of certain cancer treatments. This condition elevates the risk of infections,... Read more

Pathology

view channel
Image: The OvaCis Rapid Test discriminates benign from malignant epithelial ovarian cysts (Photo courtesy of INEX)

Intra-Operative POC Device Distinguishes Between Benign and Malignant Ovarian Cysts within 15 Minutes

Ovarian cysts represent a significant health issue for women globally, with up to 10% experiencing this condition at some point in their lives. These cysts form when fluid collects within a thin membrane... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.