We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
PURITAN MEDICAL

Download Mobile App




Homogeneous Assay Measures Small Dense LDL Cholesterol

By LabMedica International staff writers
Posted on 29 Dec 2010
A simple homogeneous assay has been developed to measure plasma concentrations of small dense-low density lipoprotein (SD-LDL). More...


A rapid assay suitable for autoanalyzers has been devised to measure the concentration of SD-LDL cholesterol in blood samples and is much easier to use than the complex methods that require special equipment and long assay times.

Scientists at the Showa University School of Medicine, (Tokyo, Japan), have identified suitable surfactants and phospholipases by screening for those selective for the SD-LDL fraction (density range: 1.044 kg/L - 1.063 kg/L) and for the dissociation of other lipoproteins, including large buoyant LDL (lb-LDL). They compared this technique with ultracentrifugal isolation of LDL subfractions and with heparin-magnesium precipitation assay for SD-LDL. They measured SD-LDL-C concentrations in 460 healthy, normolipidemic individuals. Small, dense LDL is a type of LDL cholesterol that is considered to be an emerging risk factor for cardiovascular disease. It is smaller and heavier than typical LDL cholesterol and can increase the risk of developing atherosclerosis.

The homogeneous method correlated excellently with ultracentrifugation for SD-LDL-C and exhibited within-run precision. The distribution of SD-LDL-C was skewed, and the central 95% of SD-LDL-C concentrations ranged from 0.24 to 0.88 mmol/L (9.4–34.0 mg/dL). The homogeneous assay SD-LDL-C results for 60 samples from healthy volunteers were compared with those obtained by ultracentrifugation. Serum samples were obtained from these individuals who had variable serum lipid levels; LDL-C range: 1.6–5.2 mmol/L (63–199 mg/dL); triglycerides range: 0.3–5.2 mmol/L (30–472 mg/dL), and high-density lipoprotein cholesterol: 0.9–2.5 mmol/L (35–97 mg/dL).

The authors concluded that the homogeneous assay allows reproducible measurement of SD-LDL-C within 10 minutes and appears promising in further investigations of the clinical significance of SD-LDL-C. The study was published in December 2010, in Clinical Chemistry.

Related Links:

Showa University



Gold Member
Troponin T QC
Troponin T Quality Control
3-Part Differential Hematology Analyzer
Swelab Alfa Plus Sampler
New
Silver Member
Quality Control Material
NATtrol Chlamydia trachomatis Positive Control
New
Gold Member
Quality Control Material
iPLEX Pro Exome QC Panel
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








DIASOURCE (A Biovendor Company)

Channels

Hematology

view channel
Image: CitoCBC is the world first cartridge-based CBC to be granted CLIA Waived status by FDA (Photo courtesy of CytoChip)

Disposable Cartridge-Based Test Delivers Rapid and Accurate CBC Results

Complete Blood Count (CBC) is one of the most commonly ordered lab tests, crucial for diagnosing diseases, monitoring therapies, and conducting routine health screenings. However, more than 90% of physician... Read more

Immunology

view channel
Image: The Volition Nu.Q nucleosome assay runs on the IDS i10 automated analyzer platform (Photo courtesy of VolitionRx)

Groundbreaking Lateral Flow Test Quantifies Nucleosomes in Whole Venous Blood in Minutes

Diagnosing immune disruptions quickly and accurately is crucial in conditions such as sepsis, where timely intervention is critical for patient survival. Traditional testing methods can be slow, expensive,... Read more

Pathology

view channel
Image: A real-time trial has shown that AI could speed cancer care (Photo courtesy of Campanella, et al., Nature Medicine)

AI Accurately Predicts Genetic Mutations from Routine Pathology Slides for Faster Cancer Care

Current cancer treatment decisions are often guided by genetic testing, which can be expensive, time-consuming, and not always available at leading hospitals. For patients with lung adenocarcinoma, a critical... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.