We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Point-of-Care CD4 Counter Evaluated

By LabMedica International staff writers
Posted on 10 Jan 2013
Print article
The measurement of the absolute CD4 T-cell count is critical in the initial evaluation and staging of persons infected human immunodeficiency virus (HIV).

However, access to the technology that allows for such measurements remains limited in many low resource settings where disease burden of HIV and acquired immunodeficiency disease syndrome (AIDS) is highest.

Scientists at MBio Diagnostics (Boulder, CO, USA) working with colleagues at the University of California (San Diego, CA, USA) evaluated the performance of a prototype point-of-care device (POC) to quantify CD4 T cells. The device combines single-use, disposable cartridges with a simple reader instrument. Based on the principle of static imaging cytometry with fluorescent immunostaining, the system utilizes a novel, laser-based illumination approach combined with MBio's proprietary planar waveguide technology.

A total of 94 participants provided venous whole blood samples and 52 capillary whole blood specimens. Study participants were HIV-infected individuals enrolled through a large primary HIV care program, at the University of California, San Diego between May 2011 and October 2011. Participants were predominantly male, of Caucasian and Hispanic race/ethnicity, with a median age of 44 years. The device, known as the Snapcount system, is a product of MBio Diagnostics and was compared with conventional flow cytometry (FACSCalibur, Becton Dickinson).

The prototype MBio system showed negligible quantitative bias relative to flow cytometry. Higher variability was observed in the capillary samples relative to venipuncture, but system precision was good for both capillary and venipuncture samples. There was also close agreement between results from the same participant when tested with two different systems, different operators, and different locations.

The authors wrote that developmental efforts are underway on a cartridge that incorporate lyophilized reagents and allows direct whole blood addition combined with a reader device. The reader device would include an integrated touch screen, internal and external quality control features, and data management software and network connectivity. The study was published in the January 2013 edition of the Journal of Immunological Methods.


Related Links:
MBio Diagnostics
University of California San Diego
Becton Dickinson

Gold Member
Serological Pipet Controller
PIPETBOY GENIUS
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Centrifuge
Hematocrit Centrifuge 7511M4
New
Immunofluorescence Analyzer
MPQuanti

Print article

Channels

Molecular Diagnostics

view channel
Image: Researcher Kanta Horie places a sample in a mass spectrometer that measures protein levels in blood plasma and other fluids (Photo courtesy of WashU Medicine)

Highly Accurate Blood Test Diagnoses Alzheimer’s and Measures Dementia Progression

Several blood tests are currently available to assist doctors in diagnosing Alzheimer's disease in individuals experiencing cognitive symptoms. However, these tests do not provide insights into the clinical... Read more

Microbiology

view channel
Image: Schematic representation illustrating the key findings of the study (Photo courtesy of UNIST)

Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours

Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.