Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

TAIGEN BIOSCIENCE CORP

Taigen Bioscience Corporation develops, manufactures, and sells nucleic acid extraction reagents, fully automated ins... read more Featured Products: More products

Download Mobile App




Commercial Molecular Assay Validated for Dengue Virus Outbreak

By LabMedica International staff writers
Posted on 28 Oct 2016
Dengue virus (DENV) is a member of the viral genus Flavivirus (family Flaviviridae) that is transmitted by mosquitos, causing endemic and epidemic outbreaks in tropical and subtropical areas.

Accurate, rapid, and early diagnosis of DENV infections is important for public health and optimal clinical care; however, the gold standard method of cell culture-dependent isolation of DENV during the acute phase of infection is time-consuming and cumbersome.

Medical scientists at the National Cheng Kung University (Tainan, Taiwan) collected serum samples from patients with suspected DENV infection between July and November 2015. Sera from patients with suspected DENV infection were screened using the one-step immunochromatographic SD BIOLINE Dengue DuoDengue NS1 Ag + Ab Combo assay. This rapid assay contains two test devices, which can simultaneously detect the levels of nonstructural protein 1(NS1) antigen, and immunoglobulin M (IgM) and IgG in a sample, respectively, within 15 minutes.

Viral ribonucleic acid (RNA) was extracted from serum samples and an extraction control sample using a QAIamp viral RNA mini kit or the automated extraction system LabTurbo Virus mini Kit in LabTurbo 48 Compact System. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of viral loads was performed using LightMix dengue virus EC kit, which is capable of identifying all four dengue serotypes.

A total of 8,989, 8,954, and 1,581 samples were subjected to NS1 antigen detection, IgM and IgG detection, and LightMix assays, respectively. The LightMix assay yielded a linear curve for viral loads (VL) between 102 and 106 copies/reaction, and the minimum detection limits for DENV serotype 1 (DENV1) and DENV2, DENV3, and DENV4 were 1, 10, and 100 focus forming units (FFU)/mL, respectively. There was 88.9% concordance between the results obtained using the NS1 antigen combo kit and by LightMix analysis, and the diagnostic sensitivity and specificity of the two methods were 89.4% and 100%, and 84.7% and 100%, respectively.

The authors concluded that the LightMix assay was effective for early diagnosis of DENV infection. The data indicated that high viral loads during primary infection in elderly patients may be a positive predictor for severe illness, and may contribute to high mortality rates. The study was published on October 12, 2016, in the journal Public Library of Science Neglected Tropical Diseases.

Related Links:
National Cheng Kung University



Gold Member
Troponin T QC
Troponin T Quality Control
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Piezoelectric Micropump
Disc Pump
New
Silver Member
HPV Molecular Controls
ZeptoMetrix® HPV Type 16, 18, 45 & 68 Molecular Controls
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Clinical Chemistry

view channel
Image: The tiny clay-based materials can be customized for a range of medical applications (Photo courtesy of Angira Roy and Sam O’Keefe)

‘Brilliantly Luminous’ Nanoscale Chemical Tool to Improve Disease Detection

Thousands of commercially available glowing molecules known as fluorophores are commonly used in medical imaging, disease detection, biomarker tagging, and chemical analysis. They are also integral in... Read more

Immunology

view channel
Image: The cancer stem cell test can accurately choose more effective treatments (Photo courtesy of University of Cincinnati)

Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer

Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more

Pathology

view channel
Image: The ready-to-use DUB enzyme assay kits accelerate routine DUB activity assays without compromising data quality (Photo courtesy of Adobe Stock)

Sensitive and Specific DUB Enzyme Assay Kits Require Minimal Setup Without Substrate Preparation

Ubiquitination and deubiquitination are two important physiological processes in the ubiquitin-proteasome system, responsible for protein degradation in cells. Deubiquitinating (DUB) enzymes contain around... Read more

Technology

view channel
Image: The HIV-1 self-testing chip will be capable of selectively detecting HIV in whole blood samples (Photo courtesy of Shutterstock)

Disposable Microchip Technology Could Selectively Detect HIV in Whole Blood Samples

As of the end of 2023, approximately 40 million people globally were living with HIV, and around 630,000 individuals died from AIDS-related illnesses that same year. Despite a substantial decline in deaths... Read more

Industry

view channel
Image: The collaboration aims to leverage Oxford Nanopore\'s sequencing platform and Cepheid\'s GeneXpert system to advance the field of sequencing for infectious diseases (Photo courtesy of Cepheid)

Cepheid and Oxford Nanopore Technologies Partner on Advancing Automated Sequencing-Based Solutions

Cepheid (Sunnyvale, CA, USA), a leading molecular diagnostics company, and Oxford Nanopore Technologies (Oxford, UK), the company behind a new generation of sequencing-based molecular analysis technologies,... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.