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Benchtop Multiplex Genetic Analyzer Helps Target, Identify, and Confirm Clinically Relevant Mutations

By LabMedica International staff writers
Posted on 17 Feb 2023

Laboratories are required to maintain an efficient lab workflow in order to ensure the integrity of results. More...

Now, a benchtop multiplex genetic analyzer empowers high-throughput testing by automating key aspects of sample preparation while providing laboratories with the flexibility to customize their extraction and sample preparation protocols.

The MassARRAY Dx Analyzer from Agena Bioscience, Inc. (San Diego, CA, USA) is a benchtop multiplex genetic analyzer that simplifies the complex clinical genetics environment with easy-to-interpret data, flexible biomarker detection and robust performance. Placed at the center of the clinical workflow, laboratories can optimize throughput, turn-around-times, and data analysis. With their growing need for marker coverage, the MassARRAY System empowers laboratories to provide targeted patient diagnostics more quickly, ultimately resulting in faster treatment decisions.

The MassARRAY System workflow combines mass spectrometry with end-point PCR, enabling highly multiplexed reactions under universal cycling conditions. After nucleic acid extraction, approximately 10-40ng of sample advances through a simple workflow with easy steps. It uses matrix-assisted laser desorption/ionization - time of flight (MALDI-TOF) mass spectrometry for the precise detection of DNA molecules. It distinguishes genetic variants by analyzing their individual mass, thus eliminating the need for fluorescence or labeling.

The MassARRAY system and exclusive SpectroCHIP consumable allow laboratories to genotype up to 50 genetic variants on each pad: up to 19,200 genetic variants across the entire chip. The MassARRAY is an ideal system for targeting 10s-100s of genetic markers per sample. The SpectroCHIP features 96 or 384 inert matrix pads evenly spaced across an array. Unique DNA analyte mixtures can be deposited onto any pad, enabling side-by-side testing of different assays for distinct biomarkers from varying sample types in a single run. The MassARRAY can meet a laboratory's testing needs with pre-designed and custom panels across the molecular diagnostics industry, enabling detection of a wide range of biomarkers including SNPs, insertions, deletions, translocations, copy number variation, and methylation.

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