World's First 5-Minute Real-Time PCR Instrument Debuts at ADLM 2024

OnsiteGene Inc. (San Diego, CA, USA) is introducing the new XDive, the world's first 5-minute real-time PCR instrument, at the Association for Diagnostics & Laboratory Medicine (ADLM) 2024 conference. This groundbreaking innovation completes 40 thermal cycles and real-time fluorescent imaging in just five minutes while maintaining sensitivity comparable to leading brands on the market.

OnsiteGene's XDive 5-minute PCR is an ultra-fast real-time PCR analysis platform that integrates high-speed thermal cycling, ultra-fast PCR reaction reagents, and real-time fluorescence data acquisition workflows. XDive can perform up to 16 ultra-fast reactions per experiment, providing moderate sample throughput and maximum working flexibility. It uses proprietary heating blocks, sensors, and algorithms to achieve ultra-fast ramp rates and superior temperature control accuracy. Its optical system consists of four fluorescence channels, which can be detected by multiplex real-time PCR analysis in minutes. XDive's unique TaqMan chemistry has been optimized for ultra-fast operating conditions. XDive reaction tubes accommodate reaction volumes on conventional PCR instruments, resulting in highly accurate and reproducible results for very ultra-fast fluorescence PCR analysis. The software structure of the system is simple and reasonable, and it is easy to operate.

OnsiteGene's original XDive 5-minute ultra-fast real-time PCR instrument can be easily integrated into new systems as an OEM module, significantly increasing productivity and flexibility, as well as reducing application costs. With OnsiteGene’s 5 minutes superfast magnetic bead nucleic acid extraction instrument (Xtractor) and reagent, users can also develop new ultra-fast real-time PCR applications on the XDive system with 16 sample throughput in as fast as 10 minutes from sample to answer for point-of-care molecular testing and other applications. The instrument has received FDA Emergency Use Authorization (EUA) for COVID-19 and Monkeypox testing using swab or saliva samples.

XDive's editable panel technology simplifies the regulatory process for developing test kits and syndromic panels, significantly reducing time, cost, and risk. The superfast master mix used in the XDive instrument is in a dried form, allowing for easy shipping and storage at room temperature with a shelf life of 6 months. OnsiteGene has also developed the XPrep module, designed to extract RNA/DNA and seamlessly load XDive cartridges. With the XPrep module, XDive instruments provide a comprehensive and flexible sample-to-answer platform with up to 96-sample throughput, up to 256 multiplexing targets, and a 30-minute turnaround time.

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