New Protocol Enables DNA Sequencing from Small or Degraded RNA Samples

A company with expertise in DNA and RNA sample preparation has joined forces with a major manufacturer of DNA sequencing equipment to develop a unique sample preparation method and high-throughput sequencing solution for small and degraded RNA samples.

454 Life Sciences (Branford, CT, USA), a subsidiary of Roche (Basel, Switzerland), and NuGEN Technologies (San Carlos, CA, USA) have developed a solution to the problem of working with limited amounts of sample material using both NuGEN's proprietary isothermal linear amplification (SPIA) products and the GS FLX Titanium cDNA Rapid Library Preparation protocol for the 454 GS Junior and GS FLX Systems for DNA sequencing.

"NuGEN's innovative sample preparation solutions provide a unique and powerful tool which will enable researchers to obtain high-quality data from their less abundant samples,” said Elizabeth Hutt, CEO of NuGEN Technologies. More...