Genotyping Chip Accelerates Workflow and Increases Throughput While Maintaining Accuracy

This microarray was designed to genotype 192 samples against 24 SNP (single nucleotide polymorphism) assays in a single run, providing 4,608 data points in just one hour.

Dynamic Array IFCs have an on-chip network of microfluidic channels, chambers, and valves that automatically assemble individual PCR reactions, decreasing the number of pipetting steps required by up to 100-fold. This accelerated workflow and increased throughput enables more data points, while the 192.24 chip retains Fluidigm's 99.75% or greater call accuracy and 99% or greater call rates.

The new chip can be read using either Fluidigm's BioMark HD (the BioMark HD system is the newest real-time PCR system developed by Fluidigm with enhanced productivity, increased reliability, and a smaller footprint) or EP1 (the EP1 system offers the most efficient system for high sample throughput SNP genotyping and other applications using end-point reading after PCR) systems.

"The Fluidigm 192.24 Dynamic Array chip enables life science researchers to achieve ultra-low cost, and rapid throughput while maintaining full assay and reagent flexibility. Providing the ability to use fewer SNP assays, and run a high number of samples is a format that is ideal for some applications in agricultural biochemistry and for GWAS (genome wide association) follow-up studies," said Gajus Worthington, Fluidigm president and CEO.

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