Genotyping Chip Accelerates Workflow and Increases Throughput While Maintaining Accuracy

Biotech and life science researchers now have the option of upgrading to an ultrafast genotyping system capable of providing 4,608 data points in just one hour.

The Fluidigm Corporation (South San Francisco, CA, USA) has introduced its new 192.24 Dynamic Array IFC (integrated fluidic circuit) array chip. This microarray was designed to genotype 192 samples against 24 SNP (single nucleotide polymorphism) assays in a single run, providing 4,608 data points in just one hour.

Dynamic Array IFCs have an on-chip network of microfluidic channels, chambers, and valves that automatically assemble individual PCR reactions, decreasing the number of pipetting steps required by up to 100-fold. This accelerated workflow and increased throughput enables more data points, while the 192.24 chip retains Fluidigm's 99.75% or greater call accuracy and 99% or greater call rates.

The new chip can be read using either Fluidigm's BioMark HD (the BioMark HD system is the newest real-time PCR system developed by Fluidigm with enhanced productivity, increased reliability, and a smaller footprint) or EP1 (the EP1 system offers the most efficient system for high sample throughput SNP genotyping and other applications using end-point reading after PCR) systems.

"The Fluidigm 192.24 Dynamic Array chip enables life science researchers to achieve ultra-low cost, and rapid throughput while maintaining full assay and reagent flexibility. Providing the ability to use fewer SNP assays, and run a high number of samples is a format that is ideal for some applications in agricultural biochemistry and for GWAS (genome wide association) follow-up studies," said Gajus Worthington, Fluidigm president and CEO.

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Fluidigm Corporation