New Products Facilitate Sample Enrichment for Next-Generation Sequencing Protocols

A new line of products that enables the enrichment of the whole exome (the part of the genome formed by exons, the coding portions of genes that are expressed) or custom regions of interest is now available for biotech researchers using next-generation technology DNA sequencing protocols.


The new Roche NimbleGen (Madison, WI, USA) "SeqCap EZ Exome Plus Library" enables researchers to create their own target enrichment discovery tools by adding up to 50 Mb of custom targets to the existing 64 Mb EZ Exome v3 products providing the ability to target and enrich up to 114 Mb of total genomic DNA.

NimbelGen’s SeqCap EZ Library is a liquid-based enrichment technology, which enables the targeted capture for subsequent sequencing of the exome or a targeted subset of genes. SeqCap EZ Library is compatible with various sequencing chemistries and with the addition of the Caliper Sciclone NGS Workstation, provides a comprehensive high-throughput target enrichment solution. Caliper Life Sciences (Hopkinton, MA, USA) is a leading provider of innovative liquid handling and microfluidic systems and has broad expertise in automating the processes involved in these core areas of genomics research including: extraction and purification, PCR reaction setup, automation of RT (real time)-PCR as well as the separation and direct detection of DNA and RNA.

In addition to the SeqCap EZ Library Roche NimbleGen has released new disease-specific target enrichment panels for clinical research applications. These include the SeqCap EZ Comprehensive Cancer Design, which covers 578 genes implicated in a wide range of common and rare cancers, and the SeqCap EZ Neurology Panel Design, which covers 256 genes associated with 87 neurological disorders, including Parkinson’s disease, Alzheimer’s disease, epilepsy, and autism.


“We are extremely excited to expand our targeted sequencing portfolio to meet the rapidly evolving needs of researchers in the field of next-generation sequencing,” said Thomas Schinecker, head of sequencing solutions at Roche NimbleGen. “Our proven industry leading performance in target enrichment technology gives us a strong foundation to offer these new solutions to researchers in basic research and translational research laboratories. In addition, we are looking forward into early next year when we will be launching new SeqCap EZ Reagents to provide customers with additional workflow convenience.”

Related Links:
Roche NimbleGen
Caliper Life Sciences