Next-Generation Sequencing Kits Provide Directional Library Generation with Ribosomal RNA Removal

New sequencing technology has been developed for end-to-end preparation of RNA sequence libraries in only one day.

Cambio (Cambridge, UK), the distributors of Epicentre products in the United Kingdom and Ireland, have added a new range of products to their portfolio. The ScriptSeq Complete kits combine Epicentre’s Ribo-Zero technology and rapid ScriptSeq v2 procedure for end-to-end prep of RNA sequence libraries in just one day.

The kits enable life scientists to rapidly generate directional, paired-end RNA-Seq libraries, usually containing less than 3% rRNA reads, from as small as 100 ng total RNA. 48 ScriptSeq Index PCR primers are available separately for preparing barcoded libraries. ScriptSeq Complete kits are currently available for mouse, human, and rat samples; however, Complete kits for plants and bacteria are expected in early 2013.

ScriptSeq Complete kits remove the need to buy separate kits for the different steps of RNA-Seq library generation, thereby reducing the risk of possible sample loss, bottlenecks, and unexpected costs. The Complete kits have been designed for a rapid, simple workflow, and are less costly than purchasing the components separately.

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