We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Image Analysis, Environmental Control and Experiment Handling via Mobile Devices for Life Science Research

By LabMedica International staff writers
Posted on 23 Jun 2014
Print article
Leica Application Suite Advanced Fluorescence (LAS AF)
Software platform (Photo courtesy of Leica Biosystems - Diagnostics Danaher)
Using a novel software platform designed for advanced life-science research in wide-field and confocal microscopy, advanced fluorescence (AF) researchers can now perform two-dimensional (2D) image analysis on multichannel experiments and completely control the environmental conditions of an experiment. Furthermore, users of wide-field systems can now connect to the acquisition station remotely anywhere at any time.

Leica Microsystems (Wetzlar, Germany) has launched the Leica Application Suite Advanced fluorescence (LAS AF), software platform in its 4.0 version. A workflow guides users step by step through the 2D image analysis from applying filters, thresholding, and binary image processing, to measurements and classification. Findings are obtained simply, rapidly, and reproducibly, even with multidimensional data sets. Each analysis step offers a set of tools that researchers can choose according to the analysis requirements. The image viewer provides immediate feedback on the applied settings. Analysis workflows can be saved as user-defined protocols and applied to further data sets at a later time. The analysis results can be saved with the experiment or exported as Excel reports together with histograms and images of intermediate steps for documentation or further analysis. For statistical purposes users can analyze several data sets of experiments in one run by simply adding the data to a batch list.

Samples with several fluorescent markers can be completely analyzed in one run by using separate analysis channels for different markers. Optionally, objects identified in different acquisition channels can be correlated, for example to count the number of DNA strand breaks per nucleus. 2D analysis can also be applied to color images, i.e., to determine the number of healthy cells in one analysis channel versus the number of abnormal cells in another analysis channel.

Knowledge of all environmental parameters is vital for researchers. The correct environmental conditions help minimize experiment failures and enable researchers to fully assess their results. With the Environmental Control module in LAS AF 4.0 users can monitor temperature, CO2 and O2 conditions at all times. They can also predefine changes of these environmental parameters during an experiment and even let the system wait until a specified temperature is reached before starting. The range control either displays a warning if user-defined limits are exceeded or, alternatively, it stops the whole experiment. All environmental parameter controls are combined in one user-interface, making it easy to keep track. In combination with the LAS AF Live Data Mode module, acquisition macros can be defined to run temperature profiles – for example to perform heat shock experiments.

On wide-field systems, the LAS AF Mobile Connection module enables researchers to stay connected to and interact with their experiments 24 hours a day, seven days a week—without the need to stay close to the imaging system. Users can remotely start, stop, or pause their experiments, capture images to identify the best time to start an experiment or display an image at a conference that has been taken seconds ago during a running experiment.

Mobile Connection works via web client or mobile device. As several users can be connected with the microscope system at the same time, they can also interact with each other, exchanging messages to discuss the most recent developments of a live experiment.

Related Links:

Leica Microsystems


Gold Member
Blood Gas Analyzer
GEM Premier 7000 with iQM3
Automated Blood Typing System
IH-500 NEXT
New
Newborn Screening Test
NeoMass AAAC 3.0
New
Vitamin B12 Test
CHORUS CLIA VIT B12
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Clinical Chemistry

view channel
Image: The new saliva-based test for heart failure measures two biomarkers in about 15 minutes (Photo courtesy of Trey Pittman)

POC Saliva Testing Device Predicts Heart Failure in 15 Minutes

Heart failure is a serious condition where the heart muscle is unable to pump sufficient oxygen-rich blood throughout the body. It ranks as a major cause of death globally and is particularly fatal for... Read more

Hematology

view channel
Image: The smartphone technology measures blood hemoglobin levels from a digital photo of the inner eyelid (Photo courtesy of Purdue University)

First-Of-Its-Kind Smartphone Technology Noninvasively Measures Blood Hemoglobin Levels at POC

Blood hemoglobin tests are among the most frequently conducted blood tests, as hemoglobin levels can provide vital insights into various health conditions. However, traditional tests are often underutilized... Read more

Immunology

view channel
Image: Under a microscope, DNA repair is visible as bright green spots (“foci”) in the blue-stained cell DNA. Orange highlights actively growing cancer cells (Photo courtesy of WEHI)

Simple Blood Test Could Detect Drug Resistance in Ovarian Cancer Patients

Every year, hundreds of thousands of women across the world are diagnosed with ovarian and breast cancer. PARP inhibitors (PARPi) therapy has been a major advancement in treating these cancers, particularly... Read more

Microbiology

view channel
Image: HNL Dimer can be a novel and potentially useful clinical tool in antibiotic stewardship in sepsis (Photo courtesy of Shutterstock)

Unique Blood Biomarker Shown to Effectively Monitor Sepsis Treatment

Sepsis remains a growing problem across the world, linked to high rates of mortality and morbidity. Timely and accurate diagnosis, along with effective supportive therapy, is essential in reducing sepsis-related... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.