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Molecular Diagnostics

Image: A mobile phone app called HandLens was designed to read and report paper strip SHERLOCK results (Photo courtesy of Anna Lachenauer, Stanford University School of Medicine)

Rapid CRISPR/Cas Assay for Diagnosis of Viral Fevers in Low-Tech Environments

Rapid diagnostic tests for Ebola and Lassa fever viruses based on the RNA-targeting CRISPR/Cas13a complex have been tested successfully under field conditions in several African countries. More...
07 Sep 2020
Image: Higher prevalence of clonal hematopoiesis found in the blood and bone marrow of older healthy volunteers (Photo courtesy of Sorbonne University).

Clonal Hematopoiesis Prevalence Determined in Healthy Donors

Clonal hematopoiesis of indeterminate potential (CHIP) is defined as the presence of a clone at more than 2% of variant allele frequency (VAF) in peripheral blood (PB) of individuals with no evidence of hematologic disease. More...
02 Sep 2020
Image: A magnified image captured by the device used to perform the STAR (single telomere absolute-length rapid) assay. Different fluorescent intensities reflect the length variations in individual telomere molecules (Photo courtesy of National University of Singapore).

Streamlined Method for Telomere Length Measurement Opens New Cancer Diagnostic Opportunities

In a novel approach to cancer diagnosis, researchers have developed a high-throughput digital real-time PCR method for rapidly measuring the absolute lengths and quantities of individual telomere molecules. More...
01 Sep 2020
Image: Scanning electron micrograph of inside the nucleus of a cancer cell, chromosomes are indicated by blue arrows and circular extrachromosomal DNA are indicated by orange arrows (Photo courtesy of Paul S. Mischel, MD, UC San Diego).

Pan-Cancer Study Reveals Extrachromosomal DNA Frequency

Extrachromosomal circular DNA (eccDNA) are circular DNA found in human, plant and animal cells in addition to chromosomal DNA. eccDNA originate from chromosomal DNA and can be from 50 base pairs to several mega-base pairs in length and encode regulatory elements and several full genes. More...
01 Sep 2020
Image: Mass cytometry by time of flight uses CyTOF technology to enable deep profiling of translational and clinical samples across a range of cell surface and intracellular markers (Photo courtesy of University of Virginia).

Adaptive Immune Cells in Ulcerative Colitis Revealed by Single-Cell Analyses

Inflammatory bowel disease encompasses a spectrum of complex intestinal disorders characterized by dysregulated innate and adaptive immune responses to gut microbiota in genetically susceptible hosts. More...
01 Sep 2020
Image: The CDC 2019 Novel Coronavirus (2019-nCoV) Real-Time Reverse Transcriptase (RT)–PCR Diagnostic Panel detects the SARS-CoV-2 virus in upper and lower respiratory specimens. The new P-BEST approach provides both an eight-fold improvement in testing efficiency as well as an eight-fold reduction in cost of PCR testing (Photo courtesy of Center for Disease Control and Prevention)

New Pooling Strategy Increases Efficiency and Reduces Cost of COVID-19 PCR Tests

A new way of manipulating samples for COVID-19 testing of asymptomatic and symptomatic individuals provides both an eight-fold increase in testing efficiency, as well as an eight-fold reduction in test costs. More...
31 Aug 2020
Image: Exosomes are 30-150 nanometer extracellular vesicles containing various molecular cargoes such as RNA and proteins. (Photo courtesy of Wikimedia Commons)

An Extracellular Vesicle-Based Liquid Biopsy for Early Cancer Detection

An early diagnostic approach for a wide range of cancers is based on liquid biopsy analysis of proteins found in or released by extracellular vesicles circulating in the blood. More...
27 Aug 2020
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Genetic Testing channel of LabMedica brings the latest in molecular genetics, cytogenetics, and epigenetics, and methods from PCR to FISH, and more.
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