We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

WERFEN

Werfen provides diagnostic instruments for critical care and hemostasis to meet the testing needs of medical professi... read more Featured Products: More products

Download Mobile App




Clinical Utility of Anti-Platelet Factor 4 ELISA Test Characterized

By LabMedica International staff writers
Posted on 29 Nov 2015
Print article
LIFECODES PF4 Enhanced and PF4 IgG anti-platelet factor 4 enzyme-linked immunosorbent assay
LIFECODES PF4 Enhanced and PF4 IgG anti-platelet factor 4 enzyme-linked immunosorbent assay (Photo courtesy of Werfen)
Diagnosing heparin-induced thrombocytopenia, a potentially catastrophic immune-mediated disorder, continues to pose significant challenges for clinicians, as both clinical and laboratory tools lack specificity.

There is mounting evidence supporting a positive correlation between definitive heparin-induced thrombocytopenia and optical density (OD) positivity from the widely available anti-platelet factor 4 enzyme-linked immunosorbent assays (PF4 ELISAs); however, the clinical features distinguishing these patients remain poorly understood.

Scientists at the NYU Langone Medical Center (New York, NY, USA) conducted a case-controlled, retrospective chart review of patients from two large, urban academic institutions who underwent a PF4 ELISA at a central laboratory between July 1, 2009, and July 1, 2014. PF4 ELISA assay is a polyimmunoglobulin assay (anti-immunoglobulin G (IgG)/IgAIg/M) utilizing 60 units of heparin in the positive control. Internal positive and negative control values were recorded for each patient’s PF4 ELISA.

The PF4 ELISA testing was performed using the LIFECODES PF4 Enhanced assay kit (Immucor GTI Diagnostics, Inc., Waukesha, WI, USA). In total, 184 negative patients (OD less than 0.7), and 121 positive patients (OD greater than 0.7), including 74 low-positive patients (0.7, OD less than 1.4) and 47 high-positive patients (OD greater than 1.4) were identified. Several clinical variables were significantly different in the negative group compared with the positive group, including hospital day, previous admission within the past three months, and the presence of a new thrombus. However, many of these variables were not different between the negative and low-positive group, and were only distinct between the negative and high-positive group. When the low-positive and high-positive groups were compared, only the 4T score was significantly different.

The authors concluded that patients with high-positive ODs represent a distinct clinical group when compared with low-positive ODs. However, no single characteristic analyzed was able to distinguish those with a low-positive PF4 ELISA OD and those with a high-positive PF4 ELISA OD. Our findings reinforce the clinical utility of the 4T score, which was the only clinical variable that significantly distinguished low-positive and high-positive groups. The study was published on November 19, 2015, in the Journal of Blood Medicine.

Related Links:

NYU Langone Medical Center 
Immucor GTI Diagnostics, Inc. 


Gold Member
Pharmacogenetics Panel
VeriDose Core Panel v2.0
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Multi-Function Pipetting Platform
apricot PP5
New
Dermatophytosis Rapid Diagnostic Kit
StrongStep Dermatophytosis Diagnostic Kit
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get complete access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Molecular Diagnostics

view channel
Image: Researcher Kanta Horie places a sample in a mass spectrometer that measures protein levels in blood plasma and other fluids (Photo courtesy of WashU Medicine)

Highly Accurate Blood Test Diagnoses Alzheimer’s and Measures Dementia Progression

Several blood tests are currently available to assist doctors in diagnosing Alzheimer's disease in individuals experiencing cognitive symptoms. However, these tests do not provide insights into the clinical... Read more

Immunology

view channel
Image: The findings were based on patients from the ADAURA clinical trial of the targeted therapy osimertinib for patients with NSCLC with EGFR-activated mutations (Photo courtesy of YSM Multimedia Team)

Post-Treatment Blood Test Could Inform Future Cancer Therapy Decisions

In the ongoing advancement of personalized medicine, a new study has provided evidence supporting the use of a tool that detects cancer-derived molecules in the blood of lung cancer patients years after... Read more

Microbiology

view channel
Image: Schematic representation illustrating the key findings of the study (Photo courtesy of UNIST)

Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours

Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.