We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Multiplex Molecular Test Developed for Three Arboviruses

By LabMedica International staff writers
Posted on 30 Jun 2016
Print article
Image: The 7500 Fast Dx Real-Time polymerase chain reaction (rt-PCR) instrument (Photo courtesy of Applied Biosystems).
Image: The 7500 Fast Dx Real-Time polymerase chain reaction (rt-PCR) instrument (Photo courtesy of Applied Biosystems).
Clinical manifestations of Zika virus, chikungunya virus and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, a multiplex real-time reverse transcription polymerase chain reaction (rt-PCR) for these viruses has been developed and evaluated.

The diagnosis of human Zika virus infections is confounded by a nonspecific clinical presentation, which overlaps substantially with that of dengue virus (DENV) and chikungunya virus (CHIKV) and by cross-reaction with DENV immunoglobulin M (IgM) and DENV nonstructural protein 1 in assays for Zika virus.

Scientists at the Stanford University School of Medicine, Stanford, CA, USA) evaluated the single-reaction multiplex rRT-PCR for Zika virus, CHIKV, and DENV (referred to as the ZCD assay) by testing clinical samples from persons with suspected cases in Nicaragua. They tested 216 samples by using the ZCD assay and the pan–DENV-CHIKV rRT-PCR, which is a validated duplex assay containing the DENV and CHIKV primers and probes used in the ZCD assay.

All rRT-PCR reactions were performed on an ABI 7500 instrument (Applied Biosystems, Foster City, CA, USA). A total of 173 samples were positive, 25 for DENV alone, 110 for CHIKV alone, or 38 for both. The ZCD assay and pan–DENV-CHIKV rRT-PCR showed very good agreement for DENV detection. The two assays demonstrated good agreement for CHIKV detection and the cycle threshold (Ct) for the 35 CHIKV discrepant samples were reached significantly later than the 113 concordant samples. Of the 56 Zika virus–positive samples in the ZCD assay, 39 were positive only for Zika virus, and 17 showed evidence of mixed infection.

The authors concluded that the single-reaction multiplex ZCD assay detected and differentiated Zika virus, CHIKV, and DENV. This assay should streamline molecular workflow and decrease test costs while improving detection of these three human arboviruses. The study was published in the July 2016 issue of the journal Emerging Infectious Diseases.

Related Links:
Stanford University School of Medicine
Applied Biosystems
Gold Member
Flocked Fiber Swabs
Puritan® Patented HydraFlock®
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Silver Member
ACTH Assay
ACTH ELISA
New
Silver Member
Verification Panels for Assay Development & QC
Seroconversion Panels

Print article

Channels

Molecular Diagnostics

view channel
Image: Researcher Kanta Horie places a sample in a mass spectrometer that measures protein levels in blood plasma and other fluids (Photo courtesy of WashU Medicine)

Highly Accurate Blood Test Diagnoses Alzheimer’s and Measures Dementia Progression

Several blood tests are currently available to assist doctors in diagnosing Alzheimer's disease in individuals experiencing cognitive symptoms. However, these tests do not provide insights into the clinical... Read more

Immunology

view channel
Image: The findings were based on patients from the ADAURA clinical trial of the targeted therapy osimertinib for patients with NSCLC with EGFR-activated mutations (Photo courtesy of YSM Multimedia Team)

Post-Treatment Blood Test Could Inform Future Cancer Therapy Decisions

In the ongoing advancement of personalized medicine, a new study has provided evidence supporting the use of a tool that detects cancer-derived molecules in the blood of lung cancer patients years after... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.