We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Proteinuria Determined by Automated Analyzer

By LabMedica International staff writers
Posted on 03 Jul 2012
Print article
A rapid, accurate, and cost-effective method for their detection of protein in urine has been developed for the autoanalyzer.

The method is a two-reagent system consists of a buffer solution and surfactants, and a second one consists of a dye solution of Erythrosin B being added to a urine sample, and the dye bound protein is measured by a spectrophotometer.

At the Juntendo University (Tokyo, Japan) nephrologists enrolled 70 outpatients who were screened for proteinuria by the dipstick test. Spot urine samples from the patients and 79 healthy volunteers were examined. Urine samples were stored at -80 °C. A solution containing a buffer agent with a pH 2.3 and surfactants and a solution of Erythrosin B are added to each urine sample. After letting the mixture stand for five minutes at 37 °C, the dye-bound protein is measured by a spectrophotometer at 546 nm using an automated analyzer.

The calibration curve was linear with human serum albumin concentration in the range of 2.4-200 mg/L. The detection limit, 2.4 mg/L was superior to conventional dye-binding methods by one order of magnitude and comparable to the turbidimetric immunoassay (TIA), which is quite expensive. There was an excellent correlation between the results given by the Erythrosin B (Wako Pure Chemical Industries Ltd., Tokyo, Japan) method and those by the TIA.

The authors concluded that the method is equal to the dipstick test in terms of time and cost savings. The reaction was completed within 5 minutes at 37 °C after mixing the test solution, and could be coupled with the 7170S automated analyzer (Hitachi, Tokyo, Japan) for rapid detection. The method was capable of determining actual samples without being influenced by foreign substances in urine such as urea, creatinine, and glucose. This method is sufficiently sensitive for the detection of trace proteinuria in early-stage of chronic kidney disease, and is directly applicable to actual samples. The study was published in the July 2012 issue of the journal Clinica Chimica Acta.

Related Links:

Juntendo University
Wako Pure Chemical Industries Ltd.
Hitachi



Gold Member
Fully Automated Cell Density/Viability Analyzer
BioProfile FAST CDV
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Myeloperoxidase Assay
IDK MPO ELISA
New
Vaginitis Test
Allplex Vaginitis Screening Assay

Print article

Channels

Molecular Diagnostics

view channel
Image: Researcher Kanta Horie places a sample in a mass spectrometer that measures protein levels in blood plasma and other fluids (Photo courtesy of WashU Medicine)

Highly Accurate Blood Test Diagnoses Alzheimer’s and Measures Dementia Progression

Several blood tests are currently available to assist doctors in diagnosing Alzheimer's disease in individuals experiencing cognitive symptoms. However, these tests do not provide insights into the clinical... Read more

Immunology

view channel
Image: The findings were based on patients from the ADAURA clinical trial of the targeted therapy osimertinib for patients with NSCLC with EGFR-activated mutations (Photo courtesy of YSM Multimedia Team)

Post-Treatment Blood Test Could Inform Future Cancer Therapy Decisions

In the ongoing advancement of personalized medicine, a new study has provided evidence supporting the use of a tool that detects cancer-derived molecules in the blood of lung cancer patients years after... Read more

Microbiology

view channel
Image: Schematic representation illustrating the key findings of the study (Photo courtesy of UNIST)

Breakthrough Diagnostic Technology Identifies Bacterial Infections with Almost 100% Accuracy within Three Hours

Rapid and precise identification of pathogenic microbes in patient samples is essential for the effective treatment of acute infectious diseases, such as sepsis. The fluorescence in situ hybridization... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.