Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

SARSTEDT AG+CO KG

Sarstedt provides laboratory and medical equipments, and develops, manufactures and sells equipment and consumables i... read more Featured Products: More products

Download Mobile App




Manual Aspiration by Monovette Reduces Hemolytic Sampling

By LabMedica International staff writers
Posted on 18 Aug 2021
Hemolytic blood samples are the number one cause for specimen rejection at emergency departments (ED). Triggered by unsuitable blood sampling material or incorrect handling and a related strong vacuum force, hemolytic samples often must be retaken.

Hemolysis refers to the release of hemoglobin and other intracellular components into the surrounding plasma due to the ruptured cell membrane of erythrocytes. The root cause of hemolysis in vitro is an improper sample drawing or, more specifically, an evolving strong vacuum force.

Clinical Laboratorians at the HFR Fribourg-Hôpital Cantonal (Villars-sur-Glâne, Switzerland) conducted a head-to-head study between January and April 2019. In the first eight weeks, all specimens were collected using BD Vacutainer Lithium-Heparin Gel tubes (Vacutainer, Becton, Dickinson and Company, Franklin Lakes, NJ, USA), in the second eight weeks, blood was taken using S-Monovette Lithium-Heparin Gel tubes (SARSTEDT AG, Nümbrecht, Germany) in aspiration mode. Specimens were categorized into five classes (0–30, 31–50, 51–75, 76–100, and 101+ mg/dL of cell-free hemoglobin) and for the statistical analyses, all samples exceeding 30 mg/dL were classified as hemolytic.

All blood samples from the emergency department were evaluated using a Cobas 6000, (Roche Diagnostics, Rotkreuz, Switzerland) a state-of-the-art analyzing system, and their Hemolysis Index (HI 1 = 1 mg of free cell hemoglobin in 1 dL blood plasma) was determined. Data were collected on 4,794 blood specimens (Vacutainer: 2,634 samples, S-Monovette: 2,160 samples).

The scientists reported that overall, 11.3 % of samples were rated as hemolytic because their concentration of hemolysis exceeded 30 mg/dL. This proportion differed considerably between specimens drawn by Vacutainer (17.0 %) and S-Monovette (4.3 %), meaning that, in proportion, there were four times as many hemolytic samples when using Vacutainer. While the percentage of non-hemolytic samples (HI of 0–30 mg/dl) was substantially higher for specimens drawn by S-Monovette (95.7 %) than Vacutainer (83.0 %), the opposite was true for all HI categories above 30 mg/dl.

The authors concluded that regarding hemolysis rates, a slow manual aspiration using S-Monovette was superior to vacuum tubes with predefined filling volumes, as demonstrated in the setting of their ED, which has important practical implications. This blood sampling process could be highly beneficial, not only from a financial point of view, but also with regards to reducing unnecessary tasks and stress for nursing staff and improving patient outcome overall. The study was published on July 28, 2021 in the journal Practical Laboratory Medicine.

Related Links:
HFR Fribourg-Hôpital Cantonal
Becton, Dickinson and Company
SARSTEDT AG
Roche Diagnostics



Gold Member
Serological Pipet Controller
PIPETBOY GENIUS
Verification Panels for Assay Development & QC
Seroconversion Panels
New
Total 25-Hydroxyvitamin D₂ & D₃ Assay
25-OH-VD Reagent Kit
New
Auto Clinical Chemistry Analyzer
cobas c 703
Read the full article by registering today, it's FREE! It's Free!
Register now for FREE to LabMedica.com and get access to news and events that shape the world of Clinical Laboratory Medicine.
  • Free digital version edition of LabMedica International sent by email on regular basis
  • Free print version of LabMedica International magazine (available only outside USA and Canada).
  • Free and unlimited access to back issues of LabMedica International in digital format
  • Free LabMedica International Newsletter sent every week containing the latest news
  • Free breaking news sent via email
  • Free access to Events Calendar
  • Free access to LinkXpress new product services
  • REGISTRATION IS FREE AND EASY!
Click here to Register








Channels

Clinical Chemistry

view channel
Image: The tiny clay-based materials can be customized for a range of medical applications (Photo courtesy of Angira Roy and Sam O’Keefe)

‘Brilliantly Luminous’ Nanoscale Chemical Tool to Improve Disease Detection

Thousands of commercially available glowing molecules known as fluorophores are commonly used in medical imaging, disease detection, biomarker tagging, and chemical analysis. They are also integral in... Read more

Immunology

view channel
Image: The cancer stem cell test can accurately choose more effective treatments (Photo courtesy of University of Cincinnati)

Stem Cell Test Predicts Treatment Outcome for Patients with Platinum-Resistant Ovarian Cancer

Epithelial ovarian cancer frequently responds to chemotherapy initially, but eventually, the tumor develops resistance to the therapy, leading to regrowth. This resistance is partially due to the activation... Read more

Microbiology

view channel
Image: The lab-in-tube assay could improve TB diagnoses in rural or resource-limited areas (Photo courtesy of Kenny Lass/Tulane University)

Handheld Device Delivers Low-Cost TB Results in Less Than One Hour

Tuberculosis (TB) remains the deadliest infectious disease globally, affecting an estimated 10 million people annually. In 2021, about 4.2 million TB cases went undiagnosed or unreported, mainly due to... Read more

Pathology

view channel
Image: The ready-to-use DUB enzyme assay kits accelerate routine DUB activity assays without compromising data quality (Photo courtesy of Adobe Stock)

Sensitive and Specific DUB Enzyme Assay Kits Require Minimal Setup Without Substrate Preparation

Ubiquitination and deubiquitination are two important physiological processes in the ubiquitin-proteasome system, responsible for protein degradation in cells. Deubiquitinating (DUB) enzymes contain around... Read more

Industry

view channel
Image: The collaboration aims to leverage Oxford Nanopore\'s sequencing platform and Cepheid\'s GeneXpert system to advance the field of sequencing for infectious diseases (Photo courtesy of Cepheid)

Cepheid and Oxford Nanopore Technologies Partner on Advancing Automated Sequencing-Based Solutions

Cepheid (Sunnyvale, CA, USA), a leading molecular diagnostics company, and Oxford Nanopore Technologies (Oxford, UK), the company behind a new generation of sequencing-based molecular analysis technologies,... Read more
Copyright © 2000-2025 Globetech Media. All rights reserved.