Millions of PCR Reactions Occur in Single Tube

Improved polymerase chain reaction (PCR) technology enables millions of PCR reactions in a single tube.

Following fragmentation using specifically selected restriction endonucleases, and denaturing of the DNA sample, a selector probe library is added. Each probe can hybridize to both ends of a targeted DNA restriction fragment; it guides the targeted fragments to form circular DNA molecules. Only circular DNA targets are amplified, ready for sequencing using any next-generation procedure, and results are highly reproducible.

The new HaloPlex Target Enrichment Kit is available exclusively in the UK from Cambio (Cambridge, United Kingdom). It represents a new concept in next-generation sequencing, reducing costs and saving up to 80 % in sample preparation time.

According to Dr. Naeem Ahmed, Cambio's technical support specialist, "This new Halo Genomics kit provides a ‘lab-in-a-tube' solution, which greatly simplifies PCR workflow, with no need for expensive instrumentation or automation. With next-generation nanoscale PCR, reactions are parallelized at the molecular level so that no separate time-consuming library preparation stage is required. For a project targeting 100 genes in 1,000 samples, HaloPlex Target Enrichment Kit reduces the total sample preparation time by as much as 80 percent. Genomic regions of interest are enriched at the same time as the introduction of sample barcoding and sequence motifs."

The HaloPlex PCR reagent kit for high performance targeted resequencing applications include studies of candidate genes involved in rare inherited diseases, pathway sequencing in matched tumor and normal samples, and clinical sequencing for BRCA gene assays.

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