Stool DNA Extraction Kit for Molecular Diagnostics Faster, Simpler

The process requires only standard clinical laboratory equipment.

Advanced technology in the kit includes the high performance E-Sphere stool enzyme, a thermostable reagent that digests fecal matter at 75 °C during the 15-minute incubation stage. A further innovation is the proprietary E-Sphere clean-up column, which purifies the DNA.

Phthisis Diagnostics (Charlottesville, VA, USA) introduced the kit at the American Society for Microbiology annual meeting, which took place during May 21-24, 2011, in New Orleans (LA, USA). The kit allows lab workers to extract DNA from human stool samples in a three-step process. Other similar kits take three times the steps, said company cofounder and president Crystal Icenhour.

The E-Sphere stool sample-preparation method allows manual DNA extraction from 12 stool samples in less than 30 minutes. The process includes only nine steps and minimizes sample contamination risk with just 2 sample transfers. Stool samples can be quickly processed within a biological safety cabinet.

The kit was developed and manufactured in the USA to International Organization for Standardization (ISO) 9001 & ISO 13485 standards in a Quality Systems Registrars (QSR) & current Good Manufacturing Practice (cGMP) compliant facility, and is designated as a general-purpose reagent.

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