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PCR Technology Helps Detect Food Pathogens and Spoilage Microorganisms

By Labmedica staff writers
Posted on 28 Apr 2008
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Image: Colored transmission electron micrograph of Salmonella enteritidis bacterium (Photo courtesy of A.B. Dowsett).
Image: Colored transmission electron micrograph of Salmonella enteritidis bacterium (Photo courtesy of A.B. Dowsett).
Advanced real-time polymerase chain reaction (PCR) technology is increasingly being used in the rapid detection of food pathogens and spoilage microorganisms. The technique allows an exponential amplification of DNA from just a few to more than a billion copies in less than one hour. This enables a significant reduction in the time taken to achieve results in comparison with conventional cultivation techniques.

The increasing use of rapid food safety testing is receiving more and more attention. The major reason for this key trend is that the food industry requires quick and accurate results. Traditional microbiological methods are relatively slow and sometimes inaccurate in comparison to new molecular biologic techniques. Fast and reliable detection systems are therefore the method of choice.

Under its foodproof brand, Biotecon Diagnostics (Potsdam, Germany) has developed real-time PCR detection kits for Enterobacteriaceae plus Enterobacter sakazakii (new), Salmonella, Listeria, Campylobacter, Escherichia coli O157, among others. The range also comprises a unique kit for the screening of beer spoilage organisms as well as genetically modified organism (GMO) testing kits.

To enhance the worldwide launch of its food safety testing systems, Biotecon Diagnostics has formed a strategic marketing alliance with the globally operating Merck (Darmstadt, Germany). Merck sells the foodproof range that has been available in almost all countries of the world since January 1, 2008. More products are expected to be forthcoming later in the year.

Dr. Kornelia Berghof-Jaeger, CEO, Biotecon Diagnostics said, "In this alliance, Merck KGaA will contribute significantly through its expertise in microbiology and its excellent customer relationships. Jointly we are able to fulfill the customer needs by introducing this new technology to the food safety testing labs around the world.”

"For Merck KGaA, this partnership means a consequential advancement in order to underline our leading position in specialty chemicals, especially in analytical reagents and test kits,” commented Wolfgang E. Schmidt, head of microbiology, hygiene, and microscopy at Merck KGaA. "Our philosophy of highest quality demands is perfectly met by the solutions offered by Biotecon Diagnostics.”


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