Engineered Tissue Effectiveness Hinges on Where It Is Grown

Tissue implants comprised of cells grown on a sponge-like scaffold have been shown in clinical trials to help heal arteries damaged by atherosclerosis and other vascular diseases. However, it has been not yet known why some implants work better than others do.

Massachusetts Institute of Technology (MIT; Cambridge, MA, USA) researchers, led by Dr. Elazer Edelman, a professor of health sciences and technology, have now shown that implanted cells’ therapeutic properties depend on their shape, which is determined by the sort of scaffold on which they are grown. The research could allow scientists to develop even more effective implants and also target many other diseases, including cancer.

“The goal is to design a material that can engineer the cells to release whatever we think is most appropriate to fight a specific disease. Then we can implant the cells and use them as an incubator,” remarked Dr. Laura Indolfi, a postdoc in Edelman’s lab and lead author of a paper on the research recently published online August 2012 in the journal Biomaterials.

Dr. Aaron Baker, a former postdoc in Dr. Edelman’s laboratory and now an assistant professor at the University of Texas at Austin (USA), is also an author of the paper. For the past 20 years, Dr. Edelman has been working on using endothelial cells grown on scaffolds made of collagen as implantable devices to treat blood vessel damage. Endothelial cells line the blood vessels and regulate important process such as tissue repair and inflammation by releasing molecules such as chemokines, small proteins that transport messages between cells.

Several of the devices have been assessed in clinical trials to treat blood vessel damage; in the new Biomaterials study, Drs. Edelman and Indolfi tried to determine what makes one such tissue scaffold more effective than another. Specifically, they were interested in comparing endothelial cells grown on flat surfaces and those grown on more porous, three-dimensional scaffolds. The cells grown on three-dimensional (3D) structures tended to be more effective at repairing damage and suppressing inflammation.

The researchers discovered that cells grown on a flat surface take on a round shape in which the cells’ structural parts form a ring around the perimeter of the cell. However, when cells are grown on a scaffold with surfaces of contact whose dimensions are similar in size to the cells, they mold to the curved surfaces, taking on a more elongated shape. In those cells, the structural elements--made of bundles of the protein actin--run parallel to each other.

Those shapes determine what types of chemokines the cells secrete once implanted into the body. In this study, the researchers focused on a chemokine known as monocyte chemotactic protein-1 (MCP1), which recruits inflammatory cells called monocytes. They discovered that the structure of the cytoskeleton appears to determine whether or not the cell turns on the inflammatory pathway that produces MCP1. The elongated cells grown on porous surfaces generated eight times less of this inflammatory chemokine than cells grown on a flat surface, and recruited five times fewer monocytes than cells grown on a flat surface. This helps the tissue implants to suppress inflammation in damaged blood vessels.

The researchers also identified biomarkers that correlate the cells’ shape, chemokine secretion, and behavior. One such parameter is the production of a focal adhesion protein, which helps cells to stick to surfaces. In cells grown on a flat surface, this adhesion protein, known as vinculin, gathers around the edges of the cell. However, in cells grown on a 3D surface, the protein is evenly distributed throughout the cell. These distribution patterns serve as molecular cues to inhibit or activate the pathway that recruits monocytes.

The findings could help scientists engineer their scaffolds to customize cells to certain applications. One goal is using implanted cells to recruit other body cells that will do a specific task, such as inducing stem cells to differentiate into a certain type of cell. “By designing the matrix before we seed the cells, we can engineer which factors they are going to secrete,” Dr. Indolfi stated.

The study should also help researchers improve on existing tissue-engineered devices and evaluate new ones, According to Dr. Edelman. “Without this kind of understanding, we can’t extend successful technologies to the next generation,” he noted.

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