We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress hp
Sign In
Advertise with Us
LGC Clinical Diagnostics

Download Mobile App




Ancient Genomes Reveal Natural Selection During Introduction of Farming in Europe

By LabMedica International staff writers
Posted on 02 Dec 2015
Print article
A new study of ancient genomes has tracked evolutionary changes associated with the introduction of agriculture into Europe about 8,500 years ago, finding particular migrations and lifestyle adaptations reflected down to the molecular level of particular genes.

Until recently, scientists could try to understand the way humans adapted genetically to changes that occurred thousands of years ago only by looking at DNA variation in today's populations. But our modern genomes contain mere echoes of the past that can't be connected to specific events. Now, an international team reports a direct analysis of natural selection by analyzing a larger set of ancient human genomes only recently made available. "It allows us to put a time and date on selection and to directly associate selection with specific environmental changes, in this case the development of agriculture and the expansion of the first farmers into Europe," said study first author Dr. Iain Mathieson of Harvard Medical School (Boston, MA, USA). By taking advantage of better DNA extraction techniques and amassing what is to date the largest collection of genome-wide datasets from ancient human remains, the team was able to identify specific genes that changed during and after the transition from hunting and gathering to farming.

Many of the variants occurred on or near genes that have been associated with height, the ability to digest lactose in adulthood, fatty acid metabolism, vitamin D levels, light skin pigmentation, and blue eye color. Two variants appear on genes that have been linked to higher risk of celiac disease but they may have been important in adapting to an early agricultural diet. Other variants were located on immune-associated genes, which made sense because "the Neolithic period involved an increase in population density, with people living close to one another and to domesticated animals," said co-senior author Dr. Wolfgang Haak of University of Adelaide and Max Planck Institute, "Although that finding did not come fully as a surprise, it was great to see the selection happening."

The work also supports the idea that Europe's first farmers came from ancient Anatolia and fills in more details about how ancient groups mixed and migrated. "It's a great mystery how present-day populations got to be the way we are today, both in terms of how our ancestors moved around and intermingled and how populations developed the adaptations that help us survive a bit better in the different environments," said co-senior-author Prof. David Reich of HMS, "Now that ancient DNA is available at the genome-wide scale and in large sample sizes, we have an extraordinary new instrument for studying these questions."

"From an archaeological perspective, it's quite amazing," said co-senior-author Prof. Ron Pinhasi of University College Dublin, "The Neolithic revolution is perhaps the most important transition in human prehistory. We now have proof that people did actually go from Anatolia into Europe and brought farming with them. For more than 40 years, people thought it was impossible to answer that question." "Second," he continued, "we now have evidence that genetic selection occurred along with the changes in lifestyle and demography, and that selection continued to happen following the transition."

This team and others have used ancient DNA in the past few years to pry more from the past by learning about the genes Neanderthals passed to humans, identifying ancestors of present-day Europeans, tracing migrations into the Americas, and probing roots of Indo-European languages. Studying natural selection, however, remained out of reach because it required more ancient genomes than were available.

"In the past year, we've had a super-exponential rise in the number of ancient samples we can study on a genome scale," said Prof. Reich, "In September 2014, we had 10 individuals. In this study, we have 230." The DNA came from remains of people who lived between 3,000 and 8,500 years ago at different sites across what are now Europe, Siberia, and Turkey. That time-span provided snapshots of genetic variation before, during, and after the agricultural revolution in Europe. Among the 230 ancient individuals were 83 who hadn't been sequenced before, including the first 26 to be gathered from the eastern Mediterranean, where warm conditions usually cause DNA to degrade.

The team used several technological advances to obtain and analyze the new genetic material. For example, they exploited a method pioneered by Prof. Pinhasi's laboratory to extract DNA from a remarkably rich source: a portion of the dense, pyramid-shaped petrous bone that houses the internal auditory organs. In some cases, the bone yielded 700 times more human DNA than could be obtained from other bones, including teeth. "That changed everything," said Prof. Pinhasi, "Higher-quality DNA meant we could analyze many more positions on the genome, perform more complex tests and simulations, and start systematically studying allele frequency across populations."

Although the authors caution that sample size remains the biggest limitation of the study, comparing the ancient genomes to one another and to those of present-day people of European ancestry revealed 12 positions on the genome where natural selection related to the introduction of farming in northern latitudes appears to have happened. "Some of those specific traits have been studied before," said Prof. Reich, "This work with ancient DNA enriches our understanding of those traits and when they appeared."

Besides the adaptations that appear to be related to diet, pigmentation, immunity, and height, the possible selective pressure on other variants was less clear. "We can guess by looking at the function of the gene, but our power is limited," said Dr. Mathieson, "It's quite frustrating." It's too early to tell whether some of the variants were themselves selected for or whether they hitched a ride with a nearby beneficial gene. The question pertains especially to variants that seem to be disadvantageous, like increased disease risk.

Being able to look at numerous positions across the genome also allowed the team to examine complex traits for the first time in ancient DNA. "We can see the evolution of height across time," said Dr. Mathieson. Researchers had noticed that people from southern Europe tend to be shorter than those from northern Europe. The new study suggests that the height differential arose both from people in the north having more ancestry from Eurasian steppe populations, who seem to have been taller, and people in the south having more ancestry from Neolithic and Chalcolithic groups from the Iberian peninsula, who seem to have been shorter. The team was not able to draw conclusions about the other complex traits it investigated: body mass index, waist-hip ratio, type 2 diabetes, inflammatory bowel disease, and lipid levels.

Prof. Reich hopes researchers will one day have thousands of ancient genomes to analyze and would like to see this type of study applied to non-Europeans and even to non-human species. Dr. Mathieson added, "It will be interesting to study selection in domesticated animals and to see if there is coevolution between them and the people who were domesticating them."

The study, by Mathieson I et al., was published online ahead of print November 23, 2015, in the journal Nature.

Related Links:

Harvard Medical School 


Gold Member
Antipsychotic TDM Assays
Saladax Antipsychotic Assays
Antipsychotic TDM AssaysSaladax Antipsychotic Assays
New
PSA Test
Human Semen Rapid Test
New
Thyroxine ELISA
T4 ELISA

Print article

Channels

Clinical Chemistry

view channel
Image: The new saliva-based test for heart failure measures two biomarkers in about 15 minutes (Photo courtesy of Trey Pittman)

POC Saliva Testing Device Predicts Heart Failure in 15 Minutes

Heart failure is a serious condition where the heart muscle is unable to pump sufficient oxygen-rich blood throughout the body. It ranks as a major cause of death globally and is particularly fatal for... Read more

Hematology

view channel
Image: The smartphone technology measures blood hemoglobin levels from a digital photo of the inner eyelid (Photo courtesy of Purdue University)

First-Of-Its-Kind Smartphone Technology Noninvasively Measures Blood Hemoglobin Levels at POC

Blood hemoglobin tests are among the most frequently conducted blood tests, as hemoglobin levels can provide vital insights into various health conditions. However, traditional tests are often underutilized... Read more

Immunology

view channel
Image: Under a microscope, DNA repair is visible as bright green spots (“foci”) in the blue-stained cell DNA. Orange highlights actively growing cancer cells (Photo courtesy of WEHI)

Simple Blood Test Could Detect Drug Resistance in Ovarian Cancer Patients

Every year, hundreds of thousands of women across the world are diagnosed with ovarian and breast cancer. PARP inhibitors (PARPi) therapy has been a major advancement in treating these cancers, particularly... Read more

Microbiology

view channel
Image: HNL Dimer can be a novel and potentially useful clinical tool in antibiotic stewardship in sepsis (Photo courtesy of Shutterstock)

Unique Blood Biomarker Shown to Effectively Monitor Sepsis Treatment

Sepsis remains a growing problem across the world, linked to high rates of mortality and morbidity. Timely and accurate diagnosis, along with effective supportive therapy, is essential in reducing sepsis-related... Read more
Copyright © 2000-2024 Globetech Media. All rights reserved.